ERK1 antibody (pThr202, pThr204) (Biotin)

Catalog No. ABIN6735479

This Mouse Monoclonal antibody specifically detects ERK1 in WB, ELISA, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for ERK1 antibody (pThr202, pThr204) (Biotin) (ABIN6735479)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ERK1 Antibody (Biotin)

Binding Specificity: pThr202, pThr204

Specificity: Recognizes the FLpTEpYVA motif of activated human ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38. Species cross-reactivity: mouse, rat, and canine.

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rat (Rattus)

Purification: Gel Filtration Chromatography

Immunogen: Synthetic peptide corresponding to the FLpTEpYVA motif of phosphorylated ERK1/2 (MAPK2/3). Conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: ELISA (0.05 µg/mL), ICC (1 - 10 µg/mL), IP, WB (0.5 µg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 0.09% sodium azide, G, sucrose, 40% glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Long term: -20°C, Short term: +4°C, Avoid freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Standard Gene Symbol: MAPK3, Gene Family: Protein Kinase, Gene Subfamily: MAPK, Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins